Конвертер

with open('input.txt') as f:
    content = f.readlines()
with open('output.txt', 'w') as f:
    for line in content:
        f.write('{' + line.strip() + '}\n')

HOST: "127.0.0.1"
NAME: "USERNAMEUSERNAMEUSERNAME"
PASS: "PASSWORDPASSWORDPASSWORD"
PORT: "9000"
USER: "DBUSERDBUSERDBUSER"

f1 = open("in.txt", "rt")
f2 = open("out.txt", "wt")
 
first = True
f2.write("{\n");
 
for line in f1:
    p = line.strip().split(":")
    if len(p) != 2:
        continue
        
    if not first:
        f2.write(",\n")
    else:
        first = False
 
    f2.write( '"{}":{}'.format(p[0], p[1]) )
 
f2.write("\n}\n")
 
f1.close()
f2.close()

with open(r'C:\input.txt', 'r') as reader:
    with open(r'C:\output.txt', 'w') as writter:
        writter.write('{\n')
        for elem in reader.readlines():
            writter.write('"{}"'.format(elem[0:4]) + elem[4:])
        else:
            writter.write('\n}')

import json
 
with open('in.txt') as file:
    result = {}
    for line in file:
        k, v = line.rstrip().split(':')
        result[k] = v.replace('"', '').strip()
with open('out.txt', 'wt') as file:
    json.dump(result, file)